Jean Yee Hwa Yang School of Mathematics and Statistics, University of Sydney Aspects of the Design and Analysis of Gene Expression Studies Wednesday 15th, March 14:05-14:55pm, Carslaw Building Room 373. Proper statistical design and analysis is essential to improve the efficiency and reliability of microarray experiments, from the early design and pre-processing stages to higher level analysis. In the first part of the talk, I will illustrate issues relating to design and pre-processing with a study aimed at detecting differential gene expression between selected spliceosome mutants. This data was generated from splice-specific microarrays and featured an unbalanced, nested design with minimal degree of replication. In addition, the design of these arrays provide a platform for comparing the performance of different normalization methods. In the second part of the talk, I will present a microarray experiment that aims to identify spatially differentially expressed genes within the mouse olfactory bulb. Using this data, I will illustrate some of the main steps in the analysis of gene expression data.